Our experience indicates that truncating or mutating a protein sequence by as little as one amino acid can be the difference between soluble and insoluble protein expression results.

Nexomics Biosciences uses a proprietary approach to identify domain boundaries using a combination of three kinds of data:
(i) bioinformatics-based domain boundary and disordered region prediction
(ii) limited proteolysis with detection by mass spectrometry (MS)
(iii) amide hydrogen exchange measurements detected by MS (HDX-MS)

Please inquire for additional details